NNT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NNT transfected lysate.
Western Blot
Lane 1: NNT transfected lysate ( 22.88 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NNT full-length
Host
Human
Theoretical MW (kDa)
22.88
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NNT antibody (H00023530-B01) by Western Blots.
SDS-PAGE Gel
NNT transfected lysate.
Western Blot
Lane 1: NNT transfected lysate ( 22.88 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NNT
Entrez GeneID
23530GeneBank Accession#
BC032370.1Protein Accession#
AAH32370.1Gene Name
NNT
Gene Alias
MGC126502, MGC126503
Gene Description
nicotinamide nucleotide transhydrogenase
Omim ID
607878Gene Ontology
HyperlinkGene Summary
This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq
Other Designations
NAD(P) transhydrogenase|OTTHUMP00000122433|pyridine nucleotide transhydrogenase
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