NNT monoclonal antibody (M01), clone 1D6
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant NNT.
Immunogen
NNT (AAH32370, 1 a.a. ~ 207 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (48.51 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
NNT monoclonal antibody (M01), clone 1D6 Western Blot analysis of NNT expression in HepG2 ( Cat # L019V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NNT is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to NNT on HepG2 cell. [antibody concentration 10 ug/ml] -
Gene Info — NNT
Entrez GeneID
23530GeneBank Accession#
BC032370Protein Accession#
AAH32370Gene Name
NNT
Gene Alias
MGC126502, MGC126503
Gene Description
nicotinamide nucleotide transhydrogenase
Omim ID
607878Gene Ontology
HyperlinkGene Summary
This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq
Other Designations
NAD(P) transhydrogenase|OTTHUMP00000122433|pyridine nucleotide transhydrogenase
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Interactome
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Pathway
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Publication Reference
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Ethanol intoxication increases hepatic N-lysyl protein acetylation.
Picklo MJ Sr.
Biochemical and Biophysical Research Communications 2008 Sep; 376(3):615.
Application:WB, Rat, Rat liver.
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Ethanol intoxication increases hepatic N-lysyl protein acetylation.
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