CENTB2 monoclonal antibody (M01), clone 4G3
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CENTB2.
Immunogen
CENTB2 (NP_036419, 473 a.a. ~ 581 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
DVINRVYEANVEKMGIKKPQPGQRQEKEAYIRAKYVERKFVDKYSISLSPPEQQKKFVSKSSEEKRLSISKFGPGDQVRASAQSSVRSNDSGIQQSSDDGRESLPSTVS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (94)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CENTB2 monoclonal antibody (M01), clone 4G3 Western Blot analysis of CENTB2 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of ACAP2 expression in transfected 293T cell line by ACAP2 monoclonal antibody (M01), clone 4G3.
Lane 1: ACAP2 transfected lysate (Predicted MW: 88.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of ACAP2 transfected lysate using anti-ACAP2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACAP2 monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CENTB2 is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — ACAP2
Entrez GeneID
23527GeneBank Accession#
NM_012287Protein Accession#
NP_036419Gene Name
ACAP2
Gene Alias
CENTB2, CNT-B2, KIAA0041
Gene Description
ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Omim ID
607766Gene Ontology
HyperlinkGene Summary
O
Other Designations
Arf GAP with coiled coil, ANK repeat and PH domains 2|centaurin, beta 2
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Interactome
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Pathway
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