NIFUN 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ISCU transfected lysate.
Western Blot
Lane 1: ISCU transfected lysate ( 15.3 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NIFUN full-length
Host
Human
Theoretical MW (kDa)
15.3
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NIFUN antibody (H00023479-B01) by Western Blots.
SDS-PAGE Gel
ISCU transfected lysate.
Western Blot
Lane 1: ISCU transfected lysate ( 15.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ISCU
Entrez GeneID
23479GeneBank Accession#
NM_014301Protein Accession#
NP_055116Gene Name
ISCU
Gene Alias
2310020H20Rik, HML, ISU2, MGC74517, NIFU, NIFUN, hnifU
Gene Description
iron-sulfur cluster scaffold homolog (E. coli)
Gene Ontology
HyperlinkGene Summary
Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria. [provided by RefSeq
Other Designations
IscU iron-sulfur cluster scaffold homolog|NifU-like N-terminal domain containing|iron-sulfur cluster assembly enzyme|nitrogen fixation cluster-like
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