BRD4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
BRD4 transfected lysate.
Western Blot
Lane 1: BRD4 transfected lysate ( 88.30 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-BRD4 full-length
Host
Human
Theoretical MW (kDa)
88.3
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BRD4 antibody (H00023476-D01P) by Western Blots.
SDS-PAGE Gel
BRD4 transfected lysate.
Western Blot
Lane 1: BRD4 transfected lysate ( 88.30 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — BRD4
Entrez GeneID
23476GeneBank Accession#
BC035266.1Protein Accession#
AAH35266.1Gene Name
BRD4
Gene Alias
CAP, HUNK1, HUNKI, MCAP
Gene Description
bromodomain containing 4
Omim ID
608749Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
bromodomain-containing 4|bromodomain-containing protein 4|chromosome-associated protein
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