ELA3B monoclonal antibody (M06), clone 3H3
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ELA3B expression in transfected 293T cell line by ELA3B monoclonal antibody (M06), clone 3H3.
Lane 1: ELA3B transfected lysate (Predicted MW: 29.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (53.46 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant ELA3B.
Immunogen
ELA3B (AAH05216, 19 a.a. ~ 270 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GPPSSRPSSRVVNGEDAVPYSWPWQVSLQYEKSGSFYHTCGGSLIAPDWVVTAGHCISSSWTYQVVLGEYDRAVKEGPEQVIPINSGDLFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQLASLPPAGDILPNETPCYITGWGRLYTNGPLPDKLQEALLPVVDYEHCSRWNWWGSSVKKTMVCAGGDIRSGCNGDSGGPLNCPTEDGGWQVHGVTSFVSAFGCNTRRKPTVFTRVSAFIDWIEETIASH
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (84); Rat (82)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (53.46 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ELA3B expression in transfected 293T cell line by ELA3B monoclonal antibody (M06), clone 3H3.
Lane 1: ELA3B transfected lysate (Predicted MW: 29.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
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Gene Info — ELA3B
Entrez GeneID
23436GeneBank Accession#
BC005216Protein Accession#
AAH05216Gene Name
ELA3B
Gene Alias
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Gene Description
elastase 3B, pancreatic
Gene Ontology
HyperlinkGene Summary
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. [provided by RefSeq
Other Designations
OTTHUMP00000002837|elastase 1|elastase 3B|protease E
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