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Last updated: 2017/4/23
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RYBP monoclonal antibody (M08), clone 1F4

  • Catalog # : H00023429-M08
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a full-length recombinant RYBP.
  • Immunogen:
  • RYBP (AAH36459, 1 a.a. ~ 228 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • MTMGDKKSPTRPKRQAKPATDEGFWDCSVCTFRNSAEAFKCSICDVRKGTSTRKPRINSQLVAQQVAQQYATPPPPKKEKKEKVEKQDKEKPEKDKEISPSVTKKNTNKKTKPKSDILKDPPSEANSIQSANATTKTSETNHTSRPRLKNVDRSTAQQLAVTVGNVTVIITDFKEKTRSSSTSSSTVTSSAGSEQQNQSSSGSESTDKGSSRSSTPKGDMSAVNDESF
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2a Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00023429-M08
    Western Blot detection against Immunogen (50.82 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of RYBP expression in transfected 293T cell line by RYBP monoclonal antibody (M08), clone 1F4.

    Lane 1: RYBP transfected lysate(24.8 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation of RYBP transfected lysate using anti-RYBP monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RYBP MaxPab rabbit polyclonal antibody.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged RYBP is 0.3 ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • RYBP
  • Gene Alias:
  • AAP1,DEDAF,YEAF1
  • Gene Description:
  • RING1 and YY1 binding protein
  • Other Designations:
  • YY1 and E4TF1 associated factor 1,apoptin-associating protein 1,death effector domain-associated factor,ring1 interactor RYBP
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