RYBP monoclonal antibody (M08), clone 1F4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant RYBP.
Immunogen
RYBP (AAH36459, 1 a.a. ~ 228 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTMGDKKSPTRPKRQAKPATDEGFWDCSVCTFRNSAEAFKCSICDVRKGTSTRKPRINSQLVAQQVAQQYATPPPPKKEKKEKVEKQDKEKPEKDKEISPSVTKKNTNKKTKPKSDILKDPPSEANSIQSANATTKTSETNHTSRPRLKNVDRSTAQQLAVTVGNVTVIITDFKEKTRSSSTSSSTVTSSAGSEQQNQSSSGSESTDKGSSRSSTPKGDMSAVNDESF
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (50.82 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RYBP expression in transfected 293T cell line by RYBP monoclonal antibody (M08), clone 1F4.
Lane 1: RYBP transfected lysate(24.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of RYBP transfected lysate using anti-RYBP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RYBP MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RYBP is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — RYBP
Entrez GeneID
23429GeneBank Accession#
BC036459Protein Accession#
AAH36459Gene Name
RYBP
Gene Alias
AAP1, DEDAF, YEAF1
Gene Description
RING1 and YY1 binding protein
Omim ID
607535Gene Ontology
HyperlinkOther Designations
YY1 and E4TF1 associated factor 1|apoptin-associating protein 1|death effector domain-associated factor|ring1 interactor RYBP
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Interactome
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Disease
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