FREQ 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
FREQ transfected lysate.
Western Blot
Lane 1: FREQ transfected lysate ( 21.01 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-FREQ full-length
Host
Human
Theoretical MW (kDa)
21.01
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FREQ antibody (H00023413-B01) by Western Blots.
SDS-PAGE Gel
FREQ transfected lysate.
Western Blot
Lane 1: FREQ transfected lysate ( 21.01 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — FREQ
Entrez GeneID
23413GeneBank Accession#
NM_014286.2Protein Accession#
NP_055101.2Gene Name
FREQ
Gene Alias
DKFZp761L1223, FLUP, NCS-1, NCS1
Gene Description
frequenin homolog (Drosophila)
Omim ID
603315Gene Ontology
HyperlinkGene Summary
This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000022356|frequenin homolog|frequenin-like ubiquitous protein|neuronal calcium sensor 1
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