SIRT3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
SIRT3 transfected lysate.
Western Blot
Lane 1: SIRT3 transfected lysate ( 28.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SIRT3 full-length
Host
Human
Theoretical MW (kDa)
28.6
Interspecies Antigen Sequence
Mouse (83); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SIRT3 antibody (H00023410-B02) by Western Blots.
SDS-PAGE Gel
SIRT3 transfected lysate.
Western Blot
Lane 1: SIRT3 transfected lysate ( 28.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SIRT3
Entrez GeneID
23410GeneBank Accession#
NM_001017524.1Protein Accession#
-Gene Name
SIRT3
Gene Alias
SIR2L3
Gene Description
sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
Omim ID
604481Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq
Other Designations
mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase|silent mating type information regulation 2, S.cerevisiae, homolog 3|sir2-like 3|sirtuin 3|sirtuin type 3
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