NEDD4L monoclonal antibody (M04), clone 1D2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of NEDD4L expression in transfected 293T cell line by NEDD4L monoclonal antibody (M04), clone 1D2.
Lane 1: NEDD4L transfected lysate (Predicted MW: 104.9 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of NEDD4L transfected lysate using anti-NEDD4L monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEDD4L monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NEDD4L is 0.3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NEDD4L.
Immunogen
NEDD4L (AAH32597, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MATGLGEPVYGLSEDEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLT
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of NEDD4L expression in transfected 293T cell line by NEDD4L monoclonal antibody (M04), clone 1D2.
Lane 1: NEDD4L transfected lysate (Predicted MW: 104.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of NEDD4L transfected lysate using anti-NEDD4L monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEDD4L monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NEDD4L is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — NEDD4L
Entrez GeneID
23327GeneBank Accession#
BC032597Protein Accession#
AAH32597Gene Name
NEDD4L
Gene Alias
FLJ33870, KIAA0439, NEDD4-2, RSP5, hNedd4-2
Gene Description
neural precursor cell expressed, developmentally down-regulated 4-like
Omim ID
606384Gene Ontology
HyperlinkGene Summary
O
Other Designations
neural precursor cell expressed, developmentally down-regulated gene 4-like|ubiquitin-protein ligase NEDD4-like|ubiquitin-protein ligase Rsp5
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Interactome
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Pathway
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Disease
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