UBR2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
UBR2 transfected lysate.
Western Blot
Lane 1: UBR2 transfected lysate ( 50 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-UBR2 full-length
Host
Human
Theoretical MW (kDa)
48.4
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-UBR2 antibody (H00023304-B01) by Western Blots.
SDS-PAGE Gel
UBR2 transfected lysate.
Western Blot
Lane 1: UBR2 transfected lysate ( 50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — UBR2
Entrez GeneID
23304GeneBank Accession#
BC064512.1Protein Accession#
-Gene Name
UBR2
Gene Alias
C6orf133, DKFZp686C08114, KIAA0349, MGC71112, RP3-392M17.3, bA49A4.1, dJ242G1.1, dJ392M17.3
Gene Description
ubiquitin protein ligase E3 component n-recognin 2
Omim ID
609134Gene Ontology
HyperlinkGene Summary
Proteolysis by the ubiquitin-proteasome system controls the concentration of many regulatory proteins. The selectivity of ubiquitylation is determined by ubiquitin E3 ligases, which recognize the substrate's destabilization signal, or degron. The E3 ligase UBR2 participates in the N-end rule pathway, which targets proteins bearing an N-terminal degron, or N-degron (Kwon et al., 2003 [PubMed 14585983]).[supplied by OMIM
Other Designations
OTTHUMP00000016403|OTTHUMP00000016405|OTTHUMP00000039767|OTTHUMP00000039768|ubiquitin ligase E3 alpha-II
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Interactome
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Disease
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