FBXO28 purified MaxPab mouse polyclonal antibody (B01P)
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More Files
- More Functions
Western Blot (Cell lysate)
FBXO28 MaxPab polyclonal antibody. Western Blot analysis of FBXO28 expression in PC-12.
Western Blot (Cell lysate)
FBXO28 MaxPab polyclonal antibody. Western Blot analysis of FBXO28 expression in NIH/3T3.
Western Blot (Cell lysate)
FBXO28 MaxPab polyclonal antibody. Western Blot analysis of FBXO28 expression in HeLa.
Western Blot (Transfected lysate)
Western Blot analysis of FBXO28 expression in transfected 293T cell line (H00023219-T01) by FBXO28 MaxPab polyclonal antibody.
Lane 1: FBXO28 transfected lysate(40.48 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to FBXO28 on HeLa cell. [antibody concentration 20 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human FBXO28 protein.
Immunogen
FBXO28 (NP_055991.1, 1 a.a. ~ 368 a.a) full-length human protein.
Sequence
MAAAAEERMAEEGGGGQGDGGSSLASGSTQRQPPPPAPQHPQPGSQALPAPALAPDQLPQNNTLVALPIVAIENILSFMSYDEISQLRLVCKRMDLVCQRMLNQGFLKVERYHNLCQKQVKAQLPRRESERRNHSLARHADILAAVETRLSLLNMTFMKYVDSNLCCFIPGKVIDEIYRVLRYVNSTRAPQRAHEVLQELRDISSMAMEYFDEKIVPILKRKLPGSDVSGRLMGSPPVPGPSAALTTMQLFSKQNPSRQEVTKLQQQVKTNGAGVTVLRREISELRTKVQEQQKQLQDQDQKLLEQTQIIGEQNARLAELERKLREVMESAVGNSSGSGQNEESPRKRKKATEAIDSLRKSKRLRNRK
Host
Mouse
Reactivity
Human, Mouse, Rat
Interspecies Antigen Sequence
Mouse (94); Rat (95)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
FBXO28 MaxPab polyclonal antibody. Western Blot analysis of FBXO28 expression in PC-12.Western Blot (Cell lysate)
FBXO28 MaxPab polyclonal antibody. Western Blot analysis of FBXO28 expression in NIH/3T3.Western Blot (Cell lysate)
FBXO28 MaxPab polyclonal antibody. Western Blot analysis of FBXO28 expression in HeLa.Western Blot (Transfected lysate)
Western Blot analysis of FBXO28 expression in transfected 293T cell line (H00023219-T01) by FBXO28 MaxPab polyclonal antibody.
Lane 1: FBXO28 transfected lysate(40.48 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to FBXO28 on HeLa cell. [antibody concentration 20 ug/ml] -
Gene Info — FBXO28
Entrez GeneID
23219GeneBank Accession#
NM_015176.1Protein Accession#
NP_055991.1Gene Name
FBXO28
Gene Alias
FLJ10766, Fbx28, KIAA0483
Gene Description
F-box protein 28
Omim ID
609100Gene Ontology
HyperlinkGene Summary
Members of the F-box protein family, such as FBXO28, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM
Other Designations
OTTHUMP00000035597
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Interactome
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Publication Reference
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SCFFBXO28-mediated self-ubiquitination of FBXO28 promotes its degradation.
Cai L, Liu L, Li L, Jia L.
Cellular Signalling 2020 Jan; 65:109440.
Application：WB-Ce, WB-Tr, Human, Huh7 cells.
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SCFFBXO28-mediated self-ubiquitination of FBXO28 promotes its degradation.
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