RGL1 monoclonal antibody (M01), clone 2D10
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RGL1.
Immunogen
RGL1 (NP_055964, 2 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
EVKPVGEPTQEVSKFKLSTKVESTGHWLVEDHVRIWEVLKTEESSIQDWGEEVEEGAVYHVTLKRVQIQQAANKGARWLGVEGDQLPPGHTVSQYETCKIRTIKAGTL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (93)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.62 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
RGL1 monoclonal antibody (M01), clone 2D10 Western Blot analysis of RGL1 expression in A-431 ( Cat # L015V1 ).Western Blot (Transfected lysate)
Western Blot analysis of RGL1 expression in transfected 293T cell line by RGL1 monoclonal antibody (M01), clone 2D10.
Lane 1: RGL1 transfected lysate(90.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to RGL1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RGL1 is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — RGL1
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Interactome
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Disease
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Publication Reference
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The anti-inflammatory TIPE2 is an inhibitor of the oncogenic Ras.
Gus-Brautbar Y, Johnson D, Zhang L, Sun H, Wang P, Zhang S, Zhang L, Chen YH.
Molecular Cell 2012 Mar; 45(5):610.
Application:WB-Ce, Mouse, Raw 264.7 cells.
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The anti-inflammatory TIPE2 is an inhibitor of the oncogenic Ras.
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