GGA3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GGA3 transfected lysate.
Western Blot
Lane 1: GGA3 transfected lysate ( 74.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GGA3 full-length
Host
Human
Theoretical MW (kDa)
74.5
Interspecies Antigen Sequence
Mouse (80); Rat (72)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GGA3 antibody (H00023163-B01) by Western Blots.
SDS-PAGE Gel
GGA3 transfected lysate.
Western Blot
Lane 1: GGA3 transfected lysate ( 74.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GGA3
Entrez GeneID
23163GeneBank Accession#
BC070044.1Protein Accession#
AAH70044.1Gene Name
GGA3
Gene Alias
KIAA0154
Gene Description
golgi associated, gamma adaptin ear containing, ARF binding protein 3
Omim ID
606006Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Alternative splicing of this gene results in two transcript variants. [provided by RefSeq
Other Designations
ADP-ribosylation factor binding protein 3|Golgi-localized, gamma ear-containing, ARF-binding protein 3
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Interactome
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Pathway
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