PEG10 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PEG10 transfected lysate.
Western Blot
Lane 1: PEG10 transfected lysate ( 37 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PEG10 full-length
Host
Human
Theoretical MW (kDa)
37
Interspecies Antigen Sequence
Mouse (65)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PEG10 antibody (H00023089-B01) by Western Blots.
SDS-PAGE Gel
PEG10 transfected lysate.
Western Blot
Lane 1: PEG10 transfected lysate ( 37 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PEG10
Entrez GeneID
23089GeneBank Accession#
NM_001040152Protein Accession#
NP_001035242.1Gene Name
PEG10
Gene Alias
Edr, HB-1, KIAA1051, MEF3L, Mar2, Mart2, RGAG3
Gene Description
paternally expressed 10
Omim ID
609810Gene Ontology
HyperlinkGene Summary
This gene includes two overlapping reading frames of the same transcript encoding distinct isoforms. The shorter isoform has a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons, and it functions in part by interacting with members of the TGF-beta receptor family. The longer isoform has the active-site DSG consensus sequence of the protease domain of pol proteins. The longer isoform is the result of -1 translational frameshifting that is also seen in some retroviruses. Expression of these two isoforms only comes from the paternal allele due to imprinting. Increased gene expression (as observed by an increase in mRNA levels) is associated with hepatocellular carcinomas. [provided by RefSeq
Other Designations
MEF3 like 1|embryonal carcinoma differentiation regulated|retrotransposon gag domain containing 3
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