TNIK monoclonal antibody (M04), clone 1E4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant TNIK.
Immunogen
TNIK (AAH55427, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MKKVTDYSSSSEESESSEEEEEDGESETHDGTVAVSDIPRLIPTGAPGSNEQYNVGMVGTHGLETSHADSFSGSISREGTLMIRETSGEKKRSGHSDSNGFAGHINLPDL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of TNIK expression in transfected 293T cell line by TNIK monoclonal antibody (M04), clone 1E4.
Lane 1: TNIK transfected lysate(60.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TNIK is 1 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of TNIK over-expressed 293 cell line, cotransfected with TNIK Validated Chimera RNAi ( Cat # H00023043-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TNIK monoclonal antibody (M04) clone 1E4 (Cat # H00023043-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — TNIK
Entrez GeneID
23043GeneBank Accession#
BC055427Protein Accession#
AAH55427Gene Name
TNIK
Gene Alias
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Gene Description
TRAF2 and NCK interacting kinase
Omim ID
610005Gene Ontology
HyperlinkGene Summary
Germinal center kinases (GCKs), such as TNIK, are characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function (Fu et al., 1999 [PubMed 10521462]).[supplied by OMIM
Other Designations
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Interactome
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Disease
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