Western blot analysis of TNIK over-expressed 293 cell line, cotransfected with TNIK Validated Chimera RNAi ( Cat # H00023043-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TNIK monoclonal antibody (M04) clone 1E4 (Cat # H00023043-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Germinal center kinases (GCKs), such as TNIK, are characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function (Fu et al., 1999 [PubMed 10521462]).[supplied by OMIM