AOF2 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AOF2 full-length
Host
Human
Theoretical MW (kDa)
96.47
Interspecies Antigen Sequence
Mouse (95); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AOF2 antibody (H00023028-B01) by Western Blots.
SDS-PAGE Gel
AOF2 transfected lysate.
Western Blot
Lane 1: AOF2 transfected lysate ( 96.47 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AOF2
Entrez GeneID
23028GeneBank Accession#
BC040194.2Protein Accession#
AAH40194.3Gene Name
AOF2
Gene Alias
BHC110, KDM1, KIAA0601, LSD1
Gene Description
amine oxidase (flavin containing) domain 2
Omim ID
609132Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
BRAF35-HDAC complex protein BHC110|FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit|lysine (K)-specific demethylase 1|lysine-specific histone demethylase 1
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