Products

Western Blot (Cell lysate)

DAAM1 monoclonal antibody (M03), clone 4H3. Western Blot analysis of DAAM1 expression in A-431 ( Cat # L015V1 ).

Western Blot (Cell lysate)

DAAM1 monoclonal antibody (M03), clone 4H3. Western Blot analysis of DAAM1 expression in PC-12 ( Cat # L012V1 ).

Western Blot (Transfected lysate)

Western Blot analysis of DAAM1 expression in transfected 293T cell line by DAAM1 monoclonal antibody (M03), clone 4H3.

Lane 1: DAAM1 transfected lysate(122.3 KDa).
Lane 2: Non-transfected lysate.

Western Blot (Recombinant protein)

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged DAAM1 is approximately 1ng/ml as a capture antibody.

ELISA

In situ Proximity Ligation Assay (Cell)

Proximity Ligation Analysis of protein-protein interactions between RHOA and DAAM1. HeLa cells were stained with anti-RHOA rabbit purified polyclonal 1:1200 and anti-DAAM1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Entrez GeneID

23002

GeneBank Accession#

NM_014992

Protein Accession#

NP_055807

Gene Name

DAAM1

Gene Alias

FLJ41657, KIAA0666

Gene Description

dishevelled associated activator of morphogenesis 1

Omim ID

606626

Gene Ontology

Hyperlink

Gene Summary

Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined. [provided by RefSeq

Other Designations

OTTHUMP00000179033|dishevelled-associated activator of morphogenesis 1