NT5C2 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NT5C2 full-length
Host
Human
Theoretical MW (kDa)
65
Interspecies Antigen Sequence
Mouse (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NT5C2 antibody (H00022978-D01P) by Western Blots.
SDS-PAGE Gel
NT5C2 transfected lysate.
Western Blot
Lane 1: NT5C2 transfected lysate ( 65.00 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NT5C2
Entrez GeneID
22978GeneBank Accession#
NM_012229.2Protein Accession#
NP_036361.1Gene Name
NT5C2
Gene Alias
GMP, NT5B, PNT5, cN-II
Gene Description
5'-nucleotidase, cytosolic II
Omim ID
600417Gene Ontology
HyperlinkGene Summary
Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.[supplied by OMIM
Other Designations
5'-nucleotidase (purine), cytosolic type B|IMP-specific 5'-NT|OTTHUMP00000059167|purine 5' nucleotidase
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Interactome
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