NT5C2 monoclonal antibody (M02), clone 3C1
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant NT5C2.
Immunogen
NT5C2 (AAH01595, 1 a.a. ~ 561 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSTSWSDRLQNAADMPANMDKHALKKYRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEEEE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (99)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (87.45 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of NT5C2 expression in transfected 293T cell line by NT5C2 monoclonal antibody (M02), clone 3C1.
Lane 1: NT5C2 transfected lysate(64.97 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to NT5C2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to NT5C2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — NT5C2
Entrez GeneID
22978GeneBank Accession#
BC001595Protein Accession#
AAH01595Gene Name
NT5C2
Gene Alias
GMP, NT5B, PNT5, cN-II
Gene Description
5'-nucleotidase, cytosolic II
Omim ID
600417Gene Ontology
HyperlinkGene Summary
Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.[supplied by OMIM
Other Designations
5'-nucleotidase (purine), cytosolic type B|IMP-specific 5'-NT|OTTHUMP00000059167|purine 5' nucleotidase
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Interactome
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Pathway
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Disease
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Publication Reference
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CD73 and cN-II regulate the cellular response to chemotherapeutic and hypoxic stress in lung adenocarcinoma cells.
Muhammad-Zawwad Raza, Octavia Cadassou, Charles Dumontet, Emeline Cros-Perrial, Lars Petter Jordheim.
Biochimica et Biophysica acta. General Subjects 2021 Jan; 1865(5):129842.
Application:WB-Tr, Human, NCI-H292 cells.
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The Psychiatric Risk Gene NT5C2 Regulates Adenosine Monophosphate-Activated Protein Kinase Signaling and Protein Translation in Human Neural Progenitor Cells.
Duarte RRR, Bachtel ND, Côtel MC, Lee SH, Selvackadunco S, Watson IA, Hovsepian GA, Troakes C, Breen GD, Nixon DF, Murray RM, Bray NJ, Eleftherianos I, Vernon AC, Powell TR, Srivastava DP.
Biological Psychiatry 2019 Jul; 86(2):120.
Application:IF, IHC-P, Human, Human brains, Human neural progenitor cells.
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SAMHD1 is a biomarker for cytarabine response and a therapeutic target in acute myeloid leukemia.
Schneider C, Oellerich T, Baldauf HM, Schwarz SM, Thomas D, Flick R, Bohnenberger H, Kaderali L, Stegmann L, Cremer A, Martin M, Lohmeyer J, Michaelis M, Hornung V, Schliemann C, Berdel WE, Hartmann W, Wardelmann E, Comoglio F, Hansmann ML, Yakunin AF, Geisslinger G, Ströbel P, Ferreirós N, Serve H, Keppler OT, Cinatl J Jr.
Nature Medicine 2016 Dec; 23(2):250.
Application:WB-Ce, Human, Acute myeloid leukemia (AML) cell lines: ML2, Molm13, Mono Mac6, MV4-11, NB4, HEL, HL-60, KG-1, OCI-AML2, OCI-AML3, PL21, SIG-M5, THP-1 cells.
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Cell proliferation and drug sensitivity of human glioblastoma cells are altered by the stable modulation of cytosolic 5'-nucleotidase II.
Cividini F, Cros-Perrial E, Pesi R, Machon C, Allegrini S, Camici M, Dumontet C, Jordheim LP, Tozzi MG.
The International Journal of Biochemistry & Cell Biology 2015 Aug; 65:222.
Application:WB-Tr, Human, ADF cells.
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Determination of the enzymatic activity of cytosolic 5'-nucleotidase cN-II in cancer cells: development of a simple analytical method and related cell line models.
Jordheim LP, Puy JY, Cros-Perrial E, Peyrottes S, Lefebvre I, Perigaud C, Dumontet C.
Analytical and Bioanalytical Chemistry 2015 Jul; 407(19):5747.
Application:WB-Tr, Human, RL, HL-60, K562, GRANTA, CCRF-CEM cells.
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Cytosolic 5-Nucleotidase II Interacts with the Leucin Rich Repeat of NLR Family Member Ipaf.
Cividini F, Tozzi MG, Galli A, Pesi R, Camici M, Dumontet C, Jordheim LP, Allegrini S
PLoS One 2015 Mar; 10(3):e0121525.
Application:IP-WB, WB, WB-Tr, Human, HEK 293T, A549-pScont, A549-pScN-II, MDA-MB-231-pScont, MDA-MB-231-pScN-II cells.
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Suppression of 5'-Nucleotidase Enzymes Promotes AMP-activated Protein Kinase (AMPK) Phosphorylation and Metabolism in Human and Mouse Skeletal Muscle.
Kulkarni SS, Karlsson HK, Szekeres F, Chibalin AV, Krook A, Zierath JR.
The Journal of Biological Chemistry 2011 Oct; 286(40):34567.
Application:WB-Tr, Human, Human myotubes.
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CD73 and cN-II regulate the cellular response to chemotherapeutic and hypoxic stress in lung adenocarcinoma cells.
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