SIRT2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
SIRT2 transfected lysate.
Western Blot
Lane 1: SIRT2 transfected lysate ( 39.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SIRT2 full-length
Host
Human
Theoretical MW (kDa)
39.54
Interspecies Antigen Sequence
Mouse (87); Rat (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SIRT2 antibody (H00022933-B01) by Western Blots.
SDS-PAGE Gel
SIRT2 transfected lysate.
Western Blot
Lane 1: SIRT2 transfected lysate ( 39.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SIRT2
Entrez GeneID
22933GeneBank Accession#
NM_030593Protein Accession#
NP_085096Gene Name
SIRT2
Gene Alias
SIR2, SIR2L, SIR2L2
Gene Description
sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
Omim ID
604480Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene. [provided by RefSeq
Other Designations
silencing information regulator 2-like|silent information regulator 2|sir2-related protein type 2|sirtuin 2|sirtuin type 2
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