SEPHS2 monoclonal antibody (M02), clone 2G9
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SEPHS2.
Immunogen
SEPHS2 (NP_036380.2, 61 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GCKVPQEALLKLLAGLTRPDVRPPLGRGLVGGQEEASQEAGLPAGAGPSPTFPALGIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMM
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (77); Rat (79)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.53 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SEPHS2 monoclonal antibody (M02), clone 2G9 Western Blot analysis of SEPHS2 expression in K-562 ( Cat # L009V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to SEPHS2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SEPHS2 is approximately 0.03ng/ml as a capture antibody.ELISA
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Gene Info — SEPHS2
Entrez GeneID
22928GeneBank Accession#
NM_012248Protein Accession#
NP_036380.2Gene Name
SEPHS2
Gene Alias
SPS2
Gene Description
selenophosphate synthetase 2
Omim ID
606218Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem-loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop. Alternatively spliced transcripts have been identified, but their biological validity has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000045871|selenide,water dikinase 2|selenium donor protein 2|selenophosphate synthase
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Interactome
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Pathway
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