MAPRE1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MAPRE1 transfected lysate.
Western Blot
Lane 1: MAPRE1 transfected lysate ( 30 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MAPRE1 full-length
Host
Human
Theoretical MW (kDa)
29.59
Interspecies Antigen Sequence
Mouse (97); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MAPRE1 antibody (H00022919-B01) by Western Blots.
SDS-PAGE Gel
MAPRE1 transfected lysate.
Western Blot
Lane 1: MAPRE1 transfected lysate ( 30 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MAPRE1
Entrez GeneID
22919GeneBank Accession#
NM_012325Protein Accession#
NP_036457Gene Name
MAPRE1
Gene Alias
EB1, MGC117374, MGC129946
Gene Description
microtubule-associated protein, RP/EB family, member 1
Omim ID
603108Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq
Other Designations
OTTHUMP00000030608|adenomatous polyposis coli-binding protein EB1
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