ARSG 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ARSG transfected lysate.
Western Blot
Lane 1: ARSG transfected lysate ( 57.86 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ARSG full-length
Host
Human
Theoretical MW (kDa)
57.86
Interspecies Antigen Sequence
Mouse (82); Rat (83)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ARSG antibody (H00022901-B01) by Western Blots.
SDS-PAGE Gel
ARSG transfected lysate.
Western Blot
Lane 1: ARSG transfected lysate ( 57.86 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ARSG
Entrez GeneID
22901GeneBank Accession#
NM_014960.2Protein Accession#
NP_055775.2Gene Name
ARSG
Gene Alias
KIAA1001
Gene Description
arylsulfatase G
Omim ID
610008Gene Ontology
HyperlinkGene Summary
Sulfatases, such as ARSG, hydrolyze sulfate esters from sulfated steroids, carbohydrates, proteoglycans, and glycolipids. They are involved in hormone biosynthesis, modulation of cell signaling, and degradation of macromolecules. This gene, a novel arylsulfatase, has activity toward pseudosubstrates including p-nitrocatechol sulfate and 4-methylumbelliferyl sulfate. Activity is competitively inhibited by phosphate. The unprocessed protein is active as a 63-kDa monomer and demonstrates an acidic pH optimum as typically seen for lysosomal sulfatases. The protein accumulates within lysosomes and is also a glycoprotein that binds specifically to mannose 6-phosphate receptors. [provided by RefSeq
Other Designations
Arylsulfatase G
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Interactome
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Pathway
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Disease
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