DIS3 purified MaxPab mouse polyclonal antibody (B01P)
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More Files
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Western Blot (Transfected lysate)
Western Blot analysis of DIS3 expression in transfected 293T cell line (H00022894-T01) by DIS3 MaxPab polyclonal antibody.
Lane 1: KIAA1008 transfected lysate(105.38 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to KIAA1008 on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human DIS3 protein.
Immunogen
DIS3 (AAH56143.1, 1 a.a. ~ 958 a.a) full-length human protein.
Sequence
MLKSKTFLKKTRAGGVMKIVREHYLRDDIGCGAPGCAACGGAHEGPALEPQPQDPASSVCPQPHYLLPDTNVLLHQIDVLEDPAIRNVIVLQTVLQEVRNRSAPVYKRIRDVTNNQEKHFYTFTNEHHRETYVEQEQGENANDRNDRAIRVAAKWYNEHLKKMSADNQLQVIFITNDRRNKEKAIEEGIPAFTCEEYVKSLTANPELIDRLACLSEEGNEIESGKIIFSEHLPLSKLQQGIKSGTYLQGTFRASRENYLEATVWIHGDNEENKEIILQGLKHLNRAVHEDIVAVELLPKSQWVAPSSVVLHDEGQNEEDVEKEEERERMLKTAVSEKMLKPTGRVVGIIKRNWRPYCGMLSKSDIKESRRHLFTPADKRIPRIRIETRQASTLEGRRIIVAIDGWPRNSRYPNGHFVRNLGDVGEKETETEVLLLEHDVPHQPFSQAVLSFLPKMPWSITEKDMKNREDLRHLCICSVDPPGCTDIDDALHCRELENGNLEVGVHIADVSHFIRPGNALDQESARRGTTVYLCEKRIDMVPELLSSNLCSLKCDVDRLAFSCIWEMNHNAEILKTKFTKSVINSKASLTYAEAQLRIDSANMNDDITTSLRGLNKLAKILKKRRIEKGALTLSSPEVRFHMDSETHDPIDLQTKELRETNSMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYEILVKAARSRNLEIKTDTAKSLAESLDQAESPTFPYLNTLLRILATRCMMQAVYFCSGMDNDFHHYGLASPIYTHFTSPIRRYADVIVHRLLAVAIGADCTYPELTDKHKLADICKNLNFRHKMAQYAQRASVAFHTQLFFKSKGIVSEEAYILFVRKNAIVVLIPKYGLEGTVFFEEKDKPNPQLIYDDEIPSLKIEDTVFHVFDKVKVKIMLDSSNLQHQKIRMSLVEPQIPGISIPTDTSNMDLNGPKKKKMKLGK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (92)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of DIS3 expression in transfected 293T cell line (H00022894-T01) by DIS3 MaxPab polyclonal antibody.
Lane 1: KIAA1008 transfected lysate(105.38 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to KIAA1008 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — DIS3
Entrez GeneID
22894GeneBank Accession#
BC056143.1Protein Accession#
AAH56143.1Gene Name
DIS3
Gene Alias
DKFZp667L1817, EXOSC11, FLJ10484, KIAA1008, MGC33035, RP11-342J4.3, RRP44, bA555G22.1, dis3p
Gene Description
DIS3 mitotic control homolog (S. cerevisiae)
Omim ID
607533Gene Ontology
HyperlinkOther Designations
2810028N01Rik|DIS3 mitotic control|exosome component 11|mitotic control protein dis3 homolog
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Interactome
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Pathway
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Publication Reference
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Feedback from nuclear RNA on transcription promotes robust RNA concentration homeostasis in human cells.
Scott Berry, Micha Mǖller, Arpan Rai and Lucas Pelkmans.
Cell Systems 2022 Jun; 13(6):454.
Application：WB-Ce, Human, HCT-116 cells.
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Mammalian 5'-Capped MicroRNA Precursors that Generate a Single MicroRNA.
Xie M, Li M, Vilborg A, Lee N, Shu MD, Yartseva V, Sestan N, Steitz JA.
Cell 2013 Dec; 155(7):1568.
Application：WB, Human, Mouse, 293T, NIE-115 cells.
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Feedback from nuclear RNA on transcription promotes robust RNA concentration homeostasis in human cells.
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