LPHN1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
LPHN1 transfected lysate.
Western Blot
Lane 1: LPHN1 transfected lysate ( 20.8 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-LPHN1 full-length
Host
Human
Theoretical MW (kDa)
20.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-LPHN1 antibody (H00022859-D01P) by Western Blots.
SDS-PAGE Gel
LPHN1 transfected lysate.
Western Blot
Lane 1: LPHN1 transfected lysate ( 20.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — LPHN1
Entrez GeneID
22859GeneBank Accession#
BC019928Protein Accession#
AAH19928.1Gene Name
LPHN1
Gene Alias
CIRL1, CL1, LEC2
Gene Description
latrophilin 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the latrophilin subfamily of G-protein coupled receptors (GPCR). Latrophilins may function in both cell adhesion and signal transduction. In experiments with non-human species, endogenous proteolytic cleavage within a cysteine-rich GPS (G-protein-coupled-receptor proteolysis site) domain resulted in two subunits (a large extracellular N-terminal cell adhesion subunit and a subunit with substantial similarity to the secretin/calcitonin family of GPCRs) being non-covalently bound at the cell membrane. Latrophilin-1 has been shown to recruit the neurotoxin from black widow spider venom, alpha-latrotoxin, to the synapse plasma membrane. Alternative splicing results in multiple variants encoding distinct isoforms
Other Designations
calcium-independent alpha-latrotoxin receptor 1|lectomedin-2
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