RASA3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RASA3 transfected lysate.
Western Blot
Lane 1: RASA3 transfected lysate ( 95.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RASA3 full-length
Host
Human
Theoretical MW (kDa)
95.7
Interspecies Antigen Sequence
Mouse (94); Rat (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RASA3 antibody (H00022821-D01P) by Western Blots.
SDS-PAGE Gel
RASA3 transfected lysate.
Western Blot
Lane 1: RASA3 transfected lysate ( 95.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RASA3
Entrez GeneID
22821GeneBank Accession#
NM_007368Protein Accession#
NP_031394.2Gene Name
RASA3
Gene Alias
GAP1IP4BP, GAPIII, MGC46517, MGC47588
Gene Description
RAS p21 protein activator 3
Omim ID
605182Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This family member is an inositol 1,3,4,5-tetrakisphosphate-binding protein, like the closely related RAS p21 protein activator 2. The two family members have distinct pleckstrin-homology domains, with this particular member having a domain consistent with its localization to the plasma membrane. [provided by RefSeq
Other Designations
GTPase activating protein III|Ins(1,3,4,5)P4-binding protein|inositol 1,3,4,5-tetrakisphosphate-binding protein
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