NID2 monoclonal antibody (M01), clone 4G8
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NID2.
Immunogen
NID2 (NP_031387.2, 1276 a.a. ~ 1375 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (93)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of NID2 expression in transfected 293T cell line by NID2 monoclonal antibody (M01), clone 4G8.
Lane 1: NID2 transfected lysate (Predicted MW: 107.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NID2 is 1 ng/ml as a capture antibody.ELISA
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Gene Info — NID2
Entrez GeneID
22795GeneBank Accession#
NM_007361Protein Accession#
NP_031387.2Gene Name
NID2
Gene Alias
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Gene Description
nidogen 2 (osteonidogen)
Omim ID
605399Gene Ontology
HyperlinkGene Summary
Basement membranes, which are composed of type IV collagens (see MIM 120130), laminins (see LAMC1; MIM 150290), perlecan (HSPG2; MIM 142461), and nidogen (see NID1; MIM 131390), are thin pericellular protein matrices that control a large number of cellular activities, including adhesion, migration, differentiation, gene expression, and apoptosis.[supplied by OMIM
Other Designations
nidogen 2|osteonidogen
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