DDX42 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DDX42 full-length
Host
Human
Theoretical MW (kDa)
90.1
Interspecies Antigen Sequence
Mouse (95); Rat (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DDX42 antibody (H00011325-B01P) by Western Blots.
SDS-PAGE Gel
DDX42 transfected lysate.
Western Blot
Lane 1: DDX42 transfected lysate ( 90.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DDX42
Entrez GeneID
11325GeneBank Accession#
BC015505Protein Accession#
AAH15505.1Gene Name
DDX42
Gene Alias
FLJ43179, RHELP, RNAHP, SF3b125
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 42
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq
Other Designations
DEAD box polypeptide 42 protein|RNA helicase-like protein|SF3b125 DEAD-box protein
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