COPE 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
COPE transfected lysate.
Western Blot
Lane 1: COPE transfected lysate ( 34.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-COPE full-length
Host
Human
Theoretical MW (kDa)
34.5
Interspecies Antigen Sequence
Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-COPE antibody (H00011316-B03) by Western Blots.
SDS-PAGE Gel
COPE transfected lysate.
Western Blot
Lane 1: COPE transfected lysate ( 34.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — COPE
Entrez GeneID
11316GeneBank Accession#
NM_007263Protein Accession#
NP_009194.2Gene Name
COPE
Gene Alias
FLJ13241, epsilon-COP
Gene Description
coatomer protein complex, subunit epsilon
Omim ID
606942Gene Ontology
HyperlinkGene Summary
The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
coatomer epsilon subunit|epsilon coat protein|epsilon subunit of coatomer protein complex
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