VPS45A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
VPS45 transfected lysate.
Western Blot
Lane 1: VPS45 transfected lysate ( 62.81 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-VPS45A full-length
Host
Human
Theoretical MW (kDa)
62.81
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-VPS45A antibody (H00011311-B01) by Western Blots.
SDS-PAGE Gel
VPS45 transfected lysate.
Western Blot
Lane 1: VPS45 transfected lysate ( 62.81 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — VPS45
Entrez GeneID
11311GeneBank Accession#
NM_007259.3Protein Accession#
NP_009190.2Gene Name
VPS45
Gene Alias
H1, H1VPS45, VPS45A, VPS45B, VPS54A, VSP45, VSP45A
Gene Description
vacuolar protein sorting 45 homolog (S. cerevisiae)
Omim ID
610035Gene Ontology
HyperlinkGene Summary
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators. [provided by RefSeq
Other Designations
OTTHUMP00000014482|leucocyte vacuolar protein sorting 45|vacuolar protein sorting 45A
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