MGAT4B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MGAT4B transfected lysate.
Western Blot
Lane 1: MGAT4B transfected lysate ( 62.04 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MGAT4B full-length
Host
Human
Theoretical MW (kDa)
62.04
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MGAT4B antibody (H00011282-B01) by Western Blots.
SDS-PAGE Gel
MGAT4B transfected lysate.
Western Blot
Lane 1: MGAT4B transfected lysate ( 62.04 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MGAT4B
Entrez GeneID
11282GeneBank Accession#
NM_054013Protein Accession#
NP_463459Gene Name
MGAT4B
Gene Alias
GNT-IV, GNT-IVB
Gene Description
mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
Omim ID
604561Gene Ontology
HyperlinkGene Summary
This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme A, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq
Other Designations
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IV|alpha-1,3-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase|aminyltransferase|mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransfera
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Interactome
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Pathway
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Disease
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