SNF8 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human SNF8 full-length ORF ( NP_009172.2, 1 a.a. - 258 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MHRRGVGAGAIAKKKLAEAKYKERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRKNPEFRVQFQDMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLEELHQQVLKGRGKFAQDVSQDDLIRAIKKLKALGTGFGIIPVGGTYLIQSVPAELNMDHTVVLQLAEKNGYVTVSEIKASLKWETERARQVLEHLLKEGLAWLDLQAPGEAHYWLPALFTDLYSQEITAEEAREALP
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
55.3
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — SNF8
Entrez GeneID
11267GeneBank Accession#
NM_007241.2Protein Accession#
NP_009172.2Gene Name
SNF8
Gene Alias
Dot3, EAP30, VPS22
Gene Description
SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
Omim ID
610904Gene Ontology
HyperlinkGene Summary
SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM
Other Designations
EAP30 subunit of ELL complex
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Interactome
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Pathway
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