SNF8 rabbit monoclonal antibody
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Specifications
Product Description
Rabbit monoclonal antibody raised against a human SNF8 peptide using ARM Technology.
Immunogen
A synthetic peptide of human SNF8 is used for rabbit immunization.
Customer or Abnova will decide on the preferred peptide sequence.Host
Rabbit
Library Construction
Non-fusion antibody library from rabbit spleen (ARM Technology).
Expression
Overexpression vector and transfection into 293H cell line.
Reactivity
Human
Purification
Protein A
Isotype
IgG
Quality Control Testing
Antibody reactive against human SNF8 peptide by ELISA and mammalian transfected lysate by Western Blot.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Deliverable
Up to three rabbit IgG clones of 100 ug each will be delivered to customer.
Note
1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request. -
Applications
Western Blot (Transfected lysate)
ELISA
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Gene Info — SNF8
Entrez GeneID
11267GeneBank Accession#
SNF8Gene Name
SNF8
Gene Alias
Dot3, EAP30, VPS22
Gene Description
SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
Omim ID
610904Gene Ontology
HyperlinkGene Summary
SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM
Other Designations
EAP30 subunit of ELL complex
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