DCTN3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
Western Blot
Lane 1: DCTN3 transfected lysate ( 19.5 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
DCTN3 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DCTN3 full-length
Host
Human
Theoretical MW (kDa)
19.47
Interspecies Antigen Sequence
Mouse (86)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DCTN3 antibody (H00011258-B01) by Western Blots.
Western Blot
Lane 1: DCTN3 transfected lysate ( 19.5 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
DCTN3 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DCTN3
Entrez GeneID
11258GeneBank Accession#
NM_024348Protein Accession#
NP_077324Gene Name
DCTN3
Gene Alias
DCTN-22, DCTN22, MGC111190
Gene Description
dynactin 3 (p22)
Omim ID
607387Gene Ontology
HyperlinkGene Summary
This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000021292|OTTHUMP00000021293|OTTHUMP00000045394|dynactin 3|dynactin 3, isoform 1|dynactin light chain
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