CA5B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CA5B transfected lysate.
Western Blot
Lane 1: CA5B transfected lysate ( 34.98 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CA5B full-length
Host
Human
Theoretical MW (kDa)
34.98
Interspecies Antigen Sequence
Mouse (89); Rat (88)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CA5B antibody (H00011238-B01) by Western Blots.
SDS-PAGE Gel
CA5B transfected lysate.
Western Blot
Lane 1: CA5B transfected lysate ( 34.98 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CA5B
Entrez GeneID
11238GeneBank Accession#
NM_007220.3Protein Accession#
NP_009151.1Gene Name
CA5B
Gene Alias
CA-VB, MGC39962
Gene Description
carbonic anhydrase VB, mitochondrial
Omim ID
300230Gene Ontology
HyperlinkGene Summary
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. [provided by RefSeq
Other Designations
carbonic dehydratase
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