POLG2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
POLG2 transfected lysate.
Western Blot
Lane 1: POLG2 transfected lysate ( 54.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-POLG2 full-length
Host
Human
Theoretical MW (kDa)
54.9
Interspecies Antigen Sequence
Mouse (72); Rat (73)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-POLG2 antibody (H00011232-D01P) by Western Blots.
SDS-PAGE Gel
POLG2 transfected lysate.
Western Blot
Lane 1: POLG2 transfected lysate ( 54.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — POLG2
Entrez GeneID
11232GeneBank Accession#
BC009194Protein Accession#
AAH09194.1Gene Name
POLG2
Gene Alias
HP55, MTPOLB, PEOA4, POLB, POLG-BETA, POLGB
Gene Description
polymerase (DNA directed), gamma 2, accessory subunit
Gene Ontology
HyperlinkGene Summary
This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions
Other Designations
mitochondrial DNA polymerase, accessory subunit
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Interactome
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Pathway
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Disease
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