SEC63 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SEC63 full-length
Host
Human
Theoretical MW (kDa)
83.71
Interspecies Antigen Sequence
Mouse (96); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SEC63 antibody (H00011231-B01) by Western Blots.
SDS-PAGE Gel
SEC63 transfected lysate.
Western Blot
Lane 1: SEC63 transfected lysate ( 83.71 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SEC63
Entrez GeneID
11231GeneBank Accession#
NM_007214.3Protein Accession#
NP_009145.1Gene Name
SEC63
Gene Alias
ERdj2, PRO2507, SEC63L
Gene Description
SEC63 homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC62 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER. [provided by RefSeq
Other Designations
OTTHUMP00000016937|SEC63-like|SEC63-like protein
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Interactome
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