AKAP10 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
AKAP10 transfected lysate.
Western Blot
Lane 1: AKAP10 transfected lysate ( 72.93 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AKAP10 full-length
Host
Human
Theoretical MW (kDa)
72.93
Interspecies Antigen Sequence
Mouse (89); Rat (90)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AKAP10 antibody (H00011216-B01) by Western Blots.
SDS-PAGE Gel
AKAP10 transfected lysate.
Western Blot
Lane 1: AKAP10 transfected lysate ( 72.93 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AKAP10
Entrez GeneID
11216GeneBank Accession#
NM_007202.2Protein Accession#
NP_009133.2Gene Name
AKAP10
Gene Alias
D-AKAP2, MGC9414, PRKA10
Gene Description
A kinase (PRKA) anchor protein 10
Gene Ontology
HyperlinkGene Summary
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction. [provided by RefSeq
Other Designations
A-kinase anchor protein 10|dual-specificity A-kinase anchoring protein 2|mitochondrial A kinase PPKA anchor protein 10|protein kinase A anchoring protein 10
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Interactome
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Disease
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