Western blot analysis of AKAP10 over-expressed 293 cell line, cotransfected with AKAP10 Validated Chimera RNAi ( Cat # H00011216-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AKAP10 monoclonal antibody (M04), clone 8C10 (Cat # H00011216-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction. [provided by RefSeq
A-kinase anchor protein 10,dual-specificity A-kinase anchoring protein 2,mitochondrial A kinase PPKA anchor protein 10,protein kinase A anchoring protein 10