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Last updated: 2017/6/25

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CHEK2 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00011200-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-CHEK2 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 60.9
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CHEK2 antibody (H00011200-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00011200-T02
    CHEK2 transfected lysate.
    Western Blot
    QC Testing of H00011200-T02
    Lane 1: CHEK2 transfected lysate ( 60.90 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • CHEK2
  • Gene Alias:
  • CDS1,CHK2,HuCds1,LFS2,PP1425,RAD53
  • Gene Description:
  • CHK2 checkpoint homolog (S. pombe)
  • Gene Summary:
  • In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000028871,checkpoint-like protein CHK2,protein kinase CHK2,serine/threonine-protein kinase CHK2
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