SEC23IP 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SEC23IP transfected lysate.
Western Blot
Lane 1: SEC23IP transfected lysate ( 111.1 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SEC23IP full-length
Host
Human
Theoretical MW (kDa)
111.1
Interspecies Antigen Sequence
Mouse (86); Rat (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SEC23IP antibody (H00011196-B02) by Western Blots.
SDS-PAGE Gel
SEC23IP transfected lysate.
Western Blot
Lane 1: SEC23IP transfected lysate ( 111.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SEC23IP
Entrez GeneID
11196GeneBank Accession#
NM_007190Protein Accession#
NP_009121Gene Name
SEC23IP
Gene Alias
MSTP053, P125
Gene Description
SEC23 interacting protein
Gene Ontology
HyperlinkGene Summary
COPII-coated vesicles are involved in protein transport from the endoplasmic reticulum to the Golgi apparatus. The protein encoded by this gene was identified by its interaction with a mouse protein similar to yeast Sec23p, an essential component of the COPII. This protein shares significant similarity with phospholipid-modifying proteins, especially phosphatidic acid preferring-phospholipase A1. Overexpression of this protein has been shown to cause disorganization of the endoplasmic reticulum-Golgi intermediate compartment and Golgi apparatus, which suggests its role in the early secretory pathway. [provided by RefSeq
Other Designations
OTTHUMP00000020613|Sec23-interacting protein p125|phospholipase
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Interactome
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Disease
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