Western blot analysis of NUDT5 over-expressed 293 cell line, cotransfected with NUDT5 Validated Chimera RNAi ( Cat # H00011164-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NUDT5 monoclonal antibody (M04), clone 2A3 (Cat # H00011164-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Nudix hydrolases, such as NUDT5, eliminate toxic nucleotide derivatives from the cell and regulate the levels of important signaling nucleotides and their metabolites (McLennan, 1999 [PubMed 10373642]).[supplied by OMIM