LDB3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-LDB3 full-length
Host
Human
Theoretical MW (kDa)
31
Interspecies Antigen Sequence
Mouse (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-LDB3 antibody (H00011155-B01) by Western Blots.
SDS-PAGE Gel
LDB3 transfected lysate.
Western Blot
Lane 1: LDB3 transfected lysate ( 31 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — LDB3
Entrez GeneID
11155GeneBank Accession#
BC010929.2Protein Accession#
-Gene Name
LDB3
Gene Alias
CYPHER, FLJ35865, KIAA01613, KIAA0613, ORACLE, PDLIM6, ZASP, ldb3z1, ldb3z4
Gene Description
LIM domain binding 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1 and 2) have C-terminal LIM domains. [provided by RefSeq
Other Designations
OTTHUMP00000020008|OTTHUMP00000020009|PDZ and LIM domain 6
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Interactome
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Disease
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