HYPE monoclonal antibody (M02), clone 3F5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant HYPE.
Immunogen
HYPE (AAH01342, 1 a.a. ~ 449 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAVTEPKWVSVWSRFLWVTLLSMVLGSLLALLLPLGAVEEQCLAVLKGLYLLRSKPDRAQHAATKCTSPSTELSITSRGATLLVAKTKASPAGKLEARAALNQALEMKRQGKREKAQKLFMHALKMDPDFVDALTEFGIFSEEDKDIIQADYLYTRALTISPYHEKALVNRDRTLPLVEEIDQRYFSIIDSKVKKVMSIPKGNSALRRVMEETYYHHIYHTVAIEGNTLTLSEIRHILETRYAVPGKSLEEQNEVIGMHAAMKYINTTLVSRIGSVTISDVLEIHRRVLGYVDPVEAGRFRTTQVLVGHHIPPHPQDVEKQMQEFVQWLNSEEAMNLHPVEFAALAHYKLVYIHPFIDGNGRTSRLLMNLILMQAGYPPITIRKEQRSDYYHVLEAANEGDVRPFIRFIAKCTETTLDTLLFATTEYSVALPEAQPNHSGFKETLPVKP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (89); Rat (89)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (75.13 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of FICD expression in transfected 293T cell line by HYPE monoclonal antibody (M02), clone 3F5.
Lane 1: FICD transfected lysate (Predicted MW: 51.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged FICD is 1 ng/ml as a capture antibody.ELISA
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Gene Info — FICD
Entrez GeneID
11153GeneBank Accession#
BC001342Protein Accession#
AAH01342Gene Name
FICD
Gene Alias
HIP13, HYPE, MGC5623, UNQ3041
Gene Description
FIC domain containing
Gene Ontology
HyperlinkOther Designations
Huntingtin interacting protein E|fic S-phase protein cell division homolog|huntingtin interacting protein 13|huntingtin interactor protein E
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Interactome
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