JM5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
WDR45 transfected lysate
Western Blot
Lane 1: WDR45 transfected lysate ( 39.71 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-JM5 full-length
Host
Human
Theoretical MW (kDa)
39.71
Interspecies Antigen Sequence
Mouse (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-JM5 antibody (H00011152-B01) by Western Blots.
SDS-PAGE Gel
WDR45 transfected lysate
Western Blot
Lane 1: WDR45 transfected lysate ( 39.71 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — WDR45
Entrez GeneID
11152GeneBank Accession#
BC000464Protein Accession#
AAH00464Gene Name
WDR45
Gene Alias
JM5, WDRX1, WIPI-4, WIPI4
Gene Description
WD repeat domain 45
Omim ID
300526Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000032409|WD repeat domain, X-linked 1|WD45 repeat protein interacting with phosphoinositides 4
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