PWP1 monoclonal antibody (M01), clone 3A11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PWP1 expression in transfected 293T cell line by PWP1 monoclonal antibody (M01), clone 3A11.
Lane 1: PWP1 transfected lysate (Predicted MW: 55.9 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PWP1 is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PWP1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (80.85 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant PWP1.
Immunogen
PWP1 (AAH01652, 1 a.a. ~ 501 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MNRSRQVTCVAWVRCGVAKETPDKVELSKEEVKRLIAEAKEKLQEEGGGSDEEETGSPSEDGMQSARTQARPREPLEDGDPEDDRTLDDDELAEYDLDKYDEEGDPDAETLGESLLGLTVYGSNDQDPYVTLKDTEQYEREDFLIKPSDNLIVCGRAEQDQCNLDVHVYNQEEDSFYVHHDILLSAYPLSVEWLNFDPSPDDSTGNYIAVGNMTPVIEVWDLDIVDSLEPVFTLGSKLSKKKKKKGKKSSSAEGHTDAVLDLSWNKLIRNVLASASADNTVILWDMSLGKPAASLAVHTDKVQTLQFHPFEAQTLISGSYDKSVALYDCRSPDESHRMWRFSGQIERVTWNHFSPCHFLASTDDGFVYNLDARSDKPIFTLNAHNDEISGLDLSSQIKGCLVTASADKYVKIWDILGDRPSLVHSRDMKMGVLFCSSCCPDLPFIYALRGQKEGLRVWDISTVSSVNEAFGRRERLVLGSARNSSISGPFGSRSSDTPMES
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (89); Rat (89)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (80.85 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PWP1 expression in transfected 293T cell line by PWP1 monoclonal antibody (M01), clone 3A11.
Lane 1: PWP1 transfected lysate (Predicted MW: 55.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PWP1 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PWP1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PWP1
Entrez GeneID
11137GeneBank Accession#
BC001652Protein Accession#
AAH01652Gene Name
PWP1
Gene Alias
IEF-SSP-9502
Gene Description
PWP1 homolog (S. cerevisiae)
Gene Ontology
HyperlinkOther Designations
keratinocyte protein IEF SSP 9502|nuclear phosphoprotein similar to S. cerevisiae PWP1|periodic tryptophan protein 1
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Interactome
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