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CAPN10 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00011132-T02
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-CAPN10 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 74.9
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CAPN10 antibody (H00011132-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00011132-T02
    CAPN10 transfected lysate.
    Western Blot
    QC Testing of H00011132-T02
    Lane 1: CAPN10 transfected lysate ( 74.90 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • CAPN10
  • Gene Alias:
  • -
  • Gene Description:
  • calpain 10
  • Gene Summary:
  • Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM) and located within the NIDDM1 region. Multiple alternative transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000164509,calcium-activated neutral protease
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