CAPN10 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CAPN10 full-length
Host
Human
Theoretical MW (kDa)
74.03
Interspecies Antigen Sequence
Mouse (81); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CAPN10 antibody (H00011132-B01) by Western Blots.
SDS-PAGE Gel
CAPN10 transfected lysate.
Western Blot
Lane 1: CAPN10 transfected lysate ( 74.03 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CAPN10
Entrez GeneID
11132GeneBank Accession#
NM_023083.2Protein Accession#
NP_075571.1Gene Name
CAPN10
Gene Alias
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Gene Description
calpain 10
Gene Ontology
HyperlinkGene Summary
Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM) and located within the NIDDM1 region. Multiple alternative transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000164509|calcium-activated neutral protease
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Interactome
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Disease
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