ZWINT monoclonal antibody (M01), clone 6D3
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a full length recombinant ZWINT.
Immunogen
ZWINT (AAH20979, 1 a.a. ~ 277 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVRERKTGTQQELDGVFQKLGNLKQQAEQERDKLQRYQTFLQLLYTLQGKLLFPEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVNLP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (59); Rat (58)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (56.21 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ZWINT monoclonal antibody (M01), clone 6D3 Western Blot analysis of ZWINT expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ZWINT is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to ZWINT on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — ZWINT
Entrez GeneID
11130GeneBank Accession#
BC020979Protein Accession#
AAH20979Gene Name
ZWINT
Gene Alias
HZwint-1, KNTC2AP, MGC117174, ZWINT1
Gene Description
ZW10 interactor
Omim ID
609177Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000019626|OTTHUMP00000019628|human ZW10 interacting protein-1
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Interactomes
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Diseases
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